Review



passage 4  (PromoCell)


Bioz Verified Symbol PromoCell is a verified supplier
Bioz Manufacturer Symbol PromoCell manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    PromoCell passage 4
    Passage 4, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pmc13206888-143-16-26?v=PromoCell
    Average 96 stars, based on 202 article reviews
    passage 4 - by Bioz Stars, 2026-07
    96/100 stars

    Images



    Similar Products

    human hepatocellular carcinoma hepg2 passage 4 cells  (ATCC)
    99
    ATCC human hepatocellular carcinoma hepg2 passage 4 cells
    Human Hepatocellular Carcinoma Hepg2 Passage 4 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pm42018353-46-22-33?v=ATCC
    Average 99 stars, based on 1 article reviews
    human hepatocellular carcinoma hepg2 passage 4 cells - by Bioz Stars, 2026-07
    99/100 stars
    		  Buy from Supplier
    passage 4  (PromoCell)
    96
    PromoCell passage 4
    Passage 4, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pmc13206888-143-16-26?v=PromoCell
    Average 96 stars, based on 1 article reviews
    passage 4 - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier
    passage 4  (Thermo Fisher)
    94
    Thermo Fisher passage 4
    Passage 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pm41339717-431-18-23?v=Thermo+Fisher
    Average 94 stars, based on 1 article reviews
    passage 4 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    passage 3 4 huvec  (PromoCell)
    96
    PromoCell passage 3 4 huvec
    IGF-1 receptor over-expression reduces paracellular leakage and LDL-cholesterol uptake in human endothelial cells via its kinase domain. ( A ) Schema of lentiviral constructs used to achieve doxycycline-inducible WT or kinase-dead (K1003R) IGF-1R over-expression. ( B ) WT and K1003R IGF-1R protein expression are increased approximately three-fold by doxycycline. ( C ) Representative images of <t>HUVEC</t> VE-Cadherin with either WT or K1003R IGF-1R over-expression (VE-Cadherin—red, DAPI—blue). D ) VE-Cadherin junction thickness is reduced in confluent HUVEC exposed to 100 μM hydrogen peroxide over-expressing WT IGF-1R, but remains unchanged with cells over-expressing K1003R IGF-1R. ( E ) VE-Cadherin junction area is similar in confluent HUVEC over-expressing WT or K1003R IGF-1R. ( F ) Paracellular leakage of 40 kDa FITC-Dextran is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R. G ) Uptake of BODIPY-labelled LDL-cholesterol is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R (representative images on left; BODIPY—green, DAPI—blue). ( H ) Representative immunoblots of YAP/TAZ and loading controls in nuclear and cytosolic lysates, accompanied by quantification of normalized nuclear TAZ expression and nuclear:cytosolic TAZ ratio, showing reduced nuclear TAZ in HUVEC over-expressing WT vs. K1003R IGF-1R. ns denotes non-specific band in α-tubulin blot. Data expressed as mean (SEM). * P < 0.05; n = 3, 3 in a–g and n = 4, 4 in h. All statistical comparisons are made with unpaired Student’s t -tests.
    Passage 3 4 Huvec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pmc12236071-155-0-3?v=PromoCell
    Average 96 stars, based on 1 article reviews
    passage 3 4 huvec - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier
    passage 4 normal human bone marrow-derived mesenchymal stem cells (hmscs)  (Lonza)
    90
    Lonza passage 4 normal human bone marrow-derived mesenchymal stem cells (hmscs)
    IGF-1 receptor over-expression reduces paracellular leakage and LDL-cholesterol uptake in human endothelial cells via its kinase domain. ( A ) Schema of lentiviral constructs used to achieve doxycycline-inducible WT or kinase-dead (K1003R) IGF-1R over-expression. ( B ) WT and K1003R IGF-1R protein expression are increased approximately three-fold by doxycycline. ( C ) Representative images of <t>HUVEC</t> VE-Cadherin with either WT or K1003R IGF-1R over-expression (VE-Cadherin—red, DAPI—blue). D ) VE-Cadherin junction thickness is reduced in confluent HUVEC exposed to 100 μM hydrogen peroxide over-expressing WT IGF-1R, but remains unchanged with cells over-expressing K1003R IGF-1R. ( E ) VE-Cadherin junction area is similar in confluent HUVEC over-expressing WT or K1003R IGF-1R. ( F ) Paracellular leakage of 40 kDa FITC-Dextran is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R. G ) Uptake of BODIPY-labelled LDL-cholesterol is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R (representative images on left; BODIPY—green, DAPI—blue). ( H ) Representative immunoblots of YAP/TAZ and loading controls in nuclear and cytosolic lysates, accompanied by quantification of normalized nuclear TAZ expression and nuclear:cytosolic TAZ ratio, showing reduced nuclear TAZ in HUVEC over-expressing WT vs. K1003R IGF-1R. ns denotes non-specific band in α-tubulin blot. Data expressed as mean (SEM). * P < 0.05; n = 3, 3 in a–g and n = 4, 4 in h. All statistical comparisons are made with unpaired Student’s t -tests.
    Passage 4 Normal Human Bone Marrow Derived Mesenchymal Stem Cells (Hmscs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pm40108220-240-11-21?v=Lonza
    Average 90 stars, based on 1 article reviews
    passage 4 normal human bone marrow-derived mesenchymal stem cells (hmscs) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    passage 4 p4  (Eppendorf AG)
    99
    Eppendorf AG passage 4 p4
    IGF-1 receptor over-expression reduces paracellular leakage and LDL-cholesterol uptake in human endothelial cells via its kinase domain. ( A ) Schema of lentiviral constructs used to achieve doxycycline-inducible WT or kinase-dead (K1003R) IGF-1R over-expression. ( B ) WT and K1003R IGF-1R protein expression are increased approximately three-fold by doxycycline. ( C ) Representative images of <t>HUVEC</t> VE-Cadherin with either WT or K1003R IGF-1R over-expression (VE-Cadherin—red, DAPI—blue). D ) VE-Cadherin junction thickness is reduced in confluent HUVEC exposed to 100 μM hydrogen peroxide over-expressing WT IGF-1R, but remains unchanged with cells over-expressing K1003R IGF-1R. ( E ) VE-Cadherin junction area is similar in confluent HUVEC over-expressing WT or K1003R IGF-1R. ( F ) Paracellular leakage of 40 kDa FITC-Dextran is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R. G ) Uptake of BODIPY-labelled LDL-cholesterol is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R (representative images on left; BODIPY—green, DAPI—blue). ( H ) Representative immunoblots of YAP/TAZ and loading controls in nuclear and cytosolic lysates, accompanied by quantification of normalized nuclear TAZ expression and nuclear:cytosolic TAZ ratio, showing reduced nuclear TAZ in HUVEC over-expressing WT vs. K1003R IGF-1R. ns denotes non-specific band in α-tubulin blot. Data expressed as mean (SEM). * P < 0.05; n = 3, 3 in a–g and n = 4, 4 in h. All statistical comparisons are made with unpaired Student’s t -tests.
    Passage 4 P4, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pmc11858088-107-6-26?v=Eppendorf+AG
    Average 99 stars, based on 1 article reviews
    passage 4 p4 - by Bioz Stars, 2026-07
    99/100 stars
    		  Buy from Supplier
    passages 3 4  (Thermo Fisher)
    99
    Thermo Fisher passages 3 4
    IGF-1 receptor over-expression reduces paracellular leakage and LDL-cholesterol uptake in human endothelial cells via its kinase domain. ( A ) Schema of lentiviral constructs used to achieve doxycycline-inducible WT or kinase-dead (K1003R) IGF-1R over-expression. ( B ) WT and K1003R IGF-1R protein expression are increased approximately three-fold by doxycycline. ( C ) Representative images of <t>HUVEC</t> VE-Cadherin with either WT or K1003R IGF-1R over-expression (VE-Cadherin—red, DAPI—blue). D ) VE-Cadherin junction thickness is reduced in confluent HUVEC exposed to 100 μM hydrogen peroxide over-expressing WT IGF-1R, but remains unchanged with cells over-expressing K1003R IGF-1R. ( E ) VE-Cadherin junction area is similar in confluent HUVEC over-expressing WT or K1003R IGF-1R. ( F ) Paracellular leakage of 40 kDa FITC-Dextran is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R. G ) Uptake of BODIPY-labelled LDL-cholesterol is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R (representative images on left; BODIPY—green, DAPI—blue). ( H ) Representative immunoblots of YAP/TAZ and loading controls in nuclear and cytosolic lysates, accompanied by quantification of normalized nuclear TAZ expression and nuclear:cytosolic TAZ ratio, showing reduced nuclear TAZ in HUVEC over-expressing WT vs. K1003R IGF-1R. ns denotes non-specific band in α-tubulin blot. Data expressed as mean (SEM). * P < 0.05; n = 3, 3 in a–g and n = 4, 4 in h. All statistical comparisons are made with unpaired Student’s t -tests.
    Passages 3 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pm39848409-87-12-22?v=Thermo+Fisher
    Average 99 stars, based on 1 article reviews
    passages 3 4 - by Bioz Stars, 2026-07
    99/100 stars
    		  Buy from Supplier
    early passage 3 4 human lens epithelial cells b3  (ATCC)
    97
    ATCC early passage 3 4 human lens epithelial cells b3
    IGF-1 receptor over-expression reduces paracellular leakage and LDL-cholesterol uptake in human endothelial cells via its kinase domain. ( A ) Schema of lentiviral constructs used to achieve doxycycline-inducible WT or kinase-dead (K1003R) IGF-1R over-expression. ( B ) WT and K1003R IGF-1R protein expression are increased approximately three-fold by doxycycline. ( C ) Representative images of <t>HUVEC</t> VE-Cadherin with either WT or K1003R IGF-1R over-expression (VE-Cadherin—red, DAPI—blue). D ) VE-Cadherin junction thickness is reduced in confluent HUVEC exposed to 100 μM hydrogen peroxide over-expressing WT IGF-1R, but remains unchanged with cells over-expressing K1003R IGF-1R. ( E ) VE-Cadherin junction area is similar in confluent HUVEC over-expressing WT or K1003R IGF-1R. ( F ) Paracellular leakage of 40 kDa FITC-Dextran is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R. G ) Uptake of BODIPY-labelled LDL-cholesterol is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R (representative images on left; BODIPY—green, DAPI—blue). ( H ) Representative immunoblots of YAP/TAZ and loading controls in nuclear and cytosolic lysates, accompanied by quantification of normalized nuclear TAZ expression and nuclear:cytosolic TAZ ratio, showing reduced nuclear TAZ in HUVEC over-expressing WT vs. K1003R IGF-1R. ns denotes non-specific band in α-tubulin blot. Data expressed as mean (SEM). * P < 0.05; n = 3, 3 in a–g and n = 4, 4 in h. All statistical comparisons are made with unpaired Student’s t -tests.
    Early Passage 3 4 Human Lens Epithelial Cells B3, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/passage+4/pm39846689-46-0-8?v=ATCC
    Average 97 stars, based on 1 article reviews
    early passage 3 4 human lens epithelial cells b3 - by Bioz Stars, 2026-07
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    IGF-1 receptor over-expression reduces paracellular leakage and LDL-cholesterol uptake in human endothelial cells via its kinase domain. ( A ) Schema of lentiviral constructs used to achieve doxycycline-inducible WT or kinase-dead (K1003R) IGF-1R over-expression. ( B ) WT and K1003R IGF-1R protein expression are increased approximately three-fold by doxycycline. ( C ) Representative images of HUVEC VE-Cadherin with either WT or K1003R IGF-1R over-expression (VE-Cadherin—red, DAPI—blue). D ) VE-Cadherin junction thickness is reduced in confluent HUVEC exposed to 100 μM hydrogen peroxide over-expressing WT IGF-1R, but remains unchanged with cells over-expressing K1003R IGF-1R. ( E ) VE-Cadherin junction area is similar in confluent HUVEC over-expressing WT or K1003R IGF-1R. ( F ) Paracellular leakage of 40 kDa FITC-Dextran is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R. G ) Uptake of BODIPY-labelled LDL-cholesterol is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R (representative images on left; BODIPY—green, DAPI—blue). ( H ) Representative immunoblots of YAP/TAZ and loading controls in nuclear and cytosolic lysates, accompanied by quantification of normalized nuclear TAZ expression and nuclear:cytosolic TAZ ratio, showing reduced nuclear TAZ in HUVEC over-expressing WT vs. K1003R IGF-1R. ns denotes non-specific band in α-tubulin blot. Data expressed as mean (SEM). * P < 0.05; n = 3, 3 in a–g and n = 4, 4 in h. All statistical comparisons are made with unpaired Student’s t -tests.

    Journal: Cardiovascular Research

    Article Title: Endothelial insulin-like growth factor-1 signalling regulates vascular barrier function and atherogenesis

    doi: 10.1093/cvr/cvaf055

    Figure Lengend Snippet: IGF-1 receptor over-expression reduces paracellular leakage and LDL-cholesterol uptake in human endothelial cells via its kinase domain. ( A ) Schema of lentiviral constructs used to achieve doxycycline-inducible WT or kinase-dead (K1003R) IGF-1R over-expression. ( B ) WT and K1003R IGF-1R protein expression are increased approximately three-fold by doxycycline. ( C ) Representative images of HUVEC VE-Cadherin with either WT or K1003R IGF-1R over-expression (VE-Cadherin—red, DAPI—blue). D ) VE-Cadherin junction thickness is reduced in confluent HUVEC exposed to 100 μM hydrogen peroxide over-expressing WT IGF-1R, but remains unchanged with cells over-expressing K1003R IGF-1R. ( E ) VE-Cadherin junction area is similar in confluent HUVEC over-expressing WT or K1003R IGF-1R. ( F ) Paracellular leakage of 40 kDa FITC-Dextran is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R. G ) Uptake of BODIPY-labelled LDL-cholesterol is reduced in HUVEC over-expressing WT IGF-1R, but not K1003R, IGF-1R (representative images on left; BODIPY—green, DAPI—blue). ( H ) Representative immunoblots of YAP/TAZ and loading controls in nuclear and cytosolic lysates, accompanied by quantification of normalized nuclear TAZ expression and nuclear:cytosolic TAZ ratio, showing reduced nuclear TAZ in HUVEC over-expressing WT vs. K1003R IGF-1R. ns denotes non-specific band in α-tubulin blot. Data expressed as mean (SEM). * P < 0.05; n = 3, 3 in a–g and n = 4, 4 in h. All statistical comparisons are made with unpaired Student’s t -tests.

    Article Snippet: Passage 3–4 HUVEC (PromoCell) from at least three different donors were cultured in Endothelial Cell Growth Medium 2 (PromoCell) supplemented with 1× Antibiotic Antimycotic Solution (Merck) at 37°C in 5% CO 2 .

    Techniques: Over Expression, Construct, Expressing, Western Blot